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. 2019 Feb 12;11(11):979–993. doi: 10.1093/jmcb/mjz008

Figure 5.

Figure 5

ANO6 loss-of-function abolishes ADAM10-mediated shedding in Scott patient lymphocytes. Control B-cells and B-cells from Scott syndrome patients were monitored by flow cytometry for induction of PS exposure (lactadherin-FITC) and CD23 shedding. (A and B) BzATP stimulation (0.5 mM, 30 min) induced PS externalization and loss of CD23 in control cells (A, middle panel), but not in Scott patient cells (B, middle panel). (C) Fas-antibody (500 ng/ml, 6 h)-induced apoptosis led to pronounced PS exposure and concomitant CD23 loss in Scott cells. BzATP and Fas-antibody-induced CD23 shedding was abrogated in the presence of the ADAM10 inhibitor GI (3 μM). Representative pseudocolor plots of three independent experiments are shown.