Skip to main content
. 2019 Feb 12;11(11):979–993. doi: 10.1093/jmcb/mjz008

Figure 7.

Figure 7

Deletion of cationic amino acids in the ADAM10 stalk region impairs sheddase function. (A) A potential PS-binding motif (R657/K659/K660) was exchanged creating an ADAM10 stalk mutant (ADAM10-stalk Mut). (B) ADAM17/ADAM10 double-deficient HEK cells were co-transfected with BTC-AP and WT-ADAM10 (A10-WT), inactive ADAM10 (A10 E/A), or ADAM10 mutant and stimulated with ionomycin (IO, 1 μM) or melittin (Mel, 1 μM) for 30 min. AP-activity in the supernatant was calculated in relation to total (supernatant and cell pellet) AP activity and is shown in comparison to A10-WT as ‘control’. * indicates a significant increase compared to unstimulated cells; # indicates significant decrease compared to A10-WT transfected stimulated cells (P < 0.05, n = 3; ±SEM). Data were analysed by one-way analysis of variance and Bonferroni multiple comparison post hoc test.