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Zein-specific IgG titers in the sera of mice inoculated via intramuscular and subcutaneous routes with 600 µg of 290 nm zein particles at weeks 0, 1, and 3, respectively. The sera of all six mice in each group were separately evaluated three times by ELISA. Results are expressed as means of antibody titers calculated from six mice per group. All p-value calculations are based on the t-test of the two-sample equal variance hypothesis. *p < 0.05, and **p < 0.01.
