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. 2019 Dec 12;10:2922. doi: 10.3389/fimmu.2019.02922

Figure 2.

Figure 2

Knockdown of FOXO3 augments IL-10 expression in BCG-infected human primary macrophages. MDMs were transfected with FOXO3 specific siRNA (SiFOXO3) or with scramble control siRNA (siCT) for 48 h and then infected with BCG for 24 h. (A) Fold induction levels of FOXO3 and IL-10 mRNAs were assessed by RT-qPCR in BCG-infected cells. (B) IL-10 secretion by the indicated treated cells was assessed in the culture supernatants by ELISA. Knocking down of FOXO3 expression was further verified by immunoblotting. Data are shown as box and whiskers (min to max values) and are representative of three independent experiments, each one carried out in triplicate (n = 3, **p < 0.01).