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. 2019 Dec 11;10:2834. doi: 10.3389/fimmu.2019.02834

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Copyright © 2019 Wang, Liu, Tang, Yu and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Role of ITGB5 gene on ETEC F4ac adhesion to IPEC-J2 cells. (A) Standard curve for quantification of bacterial adherence to IPEC-J2 cells. (B) Reduction of ETEC F4ac adhesion after ITGB5 knockout in IPEC-J2. (C) Bacterial adhesion after ITGB5 overexpression in IPEC-J2 cells. (D) GST pull-down assays. The binding between the recombinant FaeG and ITGB5 proteins was studied using the Pierce™ GST Protein Interaction Pull-Down Kit. Western blotting with anti-GST and anti-His antibody was used for detection. Data are shown as means ± SD; n = 3. In each group, values without a common letter were significantly different (P < 0.01).