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In vitro cleavage efficiency of Cas9–sgRNA at target sites.
| Name of sgRNAs | Target sequences (5′ to 3′) | In vitro cleavage activity, % |
|---|---|---|
| ITGB5-g1 | GCCCGAAGAGGCAGGCGTAC | 0 |
| ITGB5-g2 | CGAGCGCGCAGAGCCCGAAG | 0 |
| ITGB5-g3 | CCGAAGAGGCAGGCGTACAG | 0 |
| ITGB5-g4 | GCAGGCGTACAGGGGCGCGG | 11.8 |
| ITGB5-g5 | CAGACAGTCTTCACACGAGG | 15.5 |
| ITGB5-g6 | AAGCAGACAGTCTTCACACG | 10.2 |
