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. 2019 Nov 7;5(11):e000312. doi: 10.1099/mgen.0.000312

Fig. 1.

Fig. 1.

Dot plot matrix of the alignment of the reference assembly and the hybrid Unicycler assembly using Gepard v1.40 [81]. The B. fragilis NCTC9343 (RefSeq accession number GCF_000025985.1) reference assembly derived from Sanger sequencing is on the x-axis and the hybrid Unicycler assembly on the y-axis. On this otherwise near-perfect alignment with high similarity, an 88 045 bp inversion with 100 % ID is observed at nucleotide positions 2 941 962…3 030 006 on the Unicycler assembly (2 005 742…2 093 786 on the reference sequence) (indicated by the blue arrow).