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. 2019 Nov 7;5(11):e000312. doi: 10.1099/mgen.0.000312

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© 2019 The Authors

This is an open-access article distributed under the terms of the Creative Commons Attribution License.

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Fig. 2. — Evolution of genome assemblies with added data and manual finishing. The best SPAdes assembly graphs by Unicycler with short reads only are shown on the far left. Supplying ONT reads improved the assemblies overall, but only three were circularized with singular chromosome contigs with data from the initial MinION sequencing runs. Adding additional ONT data and correcting reads with Canu did not improve assemblies for all isolates. Manual finishing was necessary to finish assemblies for three isolates. Assembly graph images generated with Bandage. Read information can be found in Table S1.