Table 3.

Hybrid Unicycler assemblies of B. fragilis CCUG4856^T

RefSeq accession GCF_000025985.1 was used as a reference. CM, Canu-corrected with option corMinCoverage=0; CO, Canu-corrected with option coroutCoverage=999; CS, Canu-corrected standard settings; OF, ONT reads filtered with Filtlong; RI, Racon polishing with Illumina reads. Unicycler performs assembly polishing with Racon (ONT reads) and Pilon. Full results are available in Table S2.

Assembly	Total length (bp)	Largest contig (bp)	Local mis-assemblies	Genome fraction (%)	Mismatches per 100 kbp	Indels per 100 kbp	K-mer-based compl. (%)	K-mer-based misjoins	ANI	Prokka CDSs	Prokka genes	Total ale score
GCF_000025985.1	5 241 700	5 205 140	0	100.000	0	0	100.00	0	100.000	4346	4439	−17071758.95
OF	5 241 602	5 205 042	3	99.970	1.11	0.65	99.96	0	99.999	4343	4436	−17245134.52
OF.RI	5 241 606	5 205 046	3	99.970	1.09	0.67	99.96	3	99.999	4345	4438	−17247815.86
OF.CS	5 241 693	5 205 133	2	99.972	0.84	0.48	99.97	1	100.000	4342	4435	−17200232.52
OF.CS.RI	5 241 698	5 205 138	2	99.972	0.88	0.52	99.96	1	100.000	4346	4439	−17206271.66
OF.CM	5 241 691	5 205 131	2	99.972	0.88	0.5	99.96	1	100.000	4343	4436	−17201292.44
OF.CM.RI	5 241 696	5 205 136	2	99.972	0.95	0.55	99.97	1	100.000	4343	4436	−17193184.79
OF.CO	5 241 693	5,205,133	2	99.972	0.84	0.48	99.97	1	100.000	4342	4435	−17200232.52
OF.CO.RI	5 241 698	5 205 138	2	99.972	0.88	0.52	99.96	1	100.000	4346	4439	−17206271.66