Fig. 3.

KRM-II-81 inhibited cortical neuronal hyperactivity in a CCI model of post-traumatic epilepsy in vivo. (A) In vivo two-photon imaging of cortical neurons in layer II/III showed great increases in integrated fluorescence (left, P < 0.001, Student’s t test) and fraction of active neurons (right, P < 0.01) in mice with CCI injury for 3 months (n = 3 in both groups). (B) Representative images of maximal projections of cortical layer II/III GCaMP6-expressing neurons at baseline and 0.5, 1, 2, and 4 hours after injection of KRM-II-81 in vivo (top row). ΔF/F traces of calcium transients of neurons corresponding to the color-circled neurons are shown on the bottom. Note the dramatic decrease in calcium activity 0.5 hours after injection of KRM-II-81. (C) There were dramatic decreases in integrated fluorescence and fraction of active cells 0.5 hours after drug injection, suggesting a decrease in cortical excitatory activity. One-way ANOVA was followed by Bonferroni test. *P < 0.05; **P < 0.01, ***P < 0.001. n = 3 mice.