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. 2019 Dec 17;12:11153–11173. doi: 10.2147/OTT.S235811

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© 2019 Kalaydina et al.

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Effect of core and terminal fucose blockade with AOL or UEA-1, respectively on DU145GemR MCTS formation for threshold diameter of ≥60µm. DU145GemR cells were grown in culture media containing 0.01 µM gemcitabine for over a year. Cells were pretreated with AOL or UEA-1 at various concentrations for 1 hr before the addition of 50 µM cyclo-RGDfK(TPP) peptide for 5 days. Cell at 10,000 were plated per well in a 96-well plate for 5 days. Spheroid volumes were measured using , where π = 3.1415, r = average radius (µm). The images were taken at 10×. The radius was measured using the scale bar in the phase-contrast image. The results are depicted as a scatter plot of data visualization using dots to represent the spheroid volume values obtained from 2–5 separate experiments. The mean ± S.E.M. for spheroid volumes is indicated for each group. The spheroid volumes were compared by ANOVA using the Holm-Sidak’s multiple comparisons test with 95% confidence with indicated adjusted p value.

Inline graphic — Effect of core and terminal fucose blockade with AOL or UEA-1, respectively on DU145GemR MCTS formation for threshold diameter of ≥60µm. DU145GemR cells were grown in culture media containing 0.01 µM gemcitabine for over a year. Cells were pretreated with AOL or UEA-1 at various concentrations for 1 hr before the addition of 50 µM cyclo-RGDfK(TPP) peptide for 5 days. Cell at 10,000 were plated per well in a 96-well plate for 5 days. Spheroid volumes were measured using , where π = 3.1415, r = average radius (µm). The images were taken at 10×. The radius was measured using the scale bar in the phase-contrast image. The results are depicted as a scatter plot of data visualization using dots to represent the spheroid volume values obtained from 2–5 separate experiments. The mean ± S.E.M. for spheroid volumes is indicated for each group. The spheroid volumes were compared by ANOVA using the Holm-Sidak’s multiple comparisons test with 95% confidence with indicated adjusted p value.