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. 2019 Dec 17;12:11153–11173. doi: 10.2147/OTT.S235811

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© 2019 Kalaydina et al.

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Comparison of the manual detection precisions of ≥40µm vs ≥60µm DU145 in the fucose lectin inhibition assay. Precision, p, is calculated using the formula, , expressed as a percentage. True positives are MCTS possess a spheroidal morphology²³^,²⁴ and diameter threshold (either ≥40 µm or ≥60 µm). False positives are MCTS that possess the spheroidal morphology but do not meet the diameter threshold. (A) Detection precision of DU145 MCTS treated with 50µM cyclo-RGDfK(TPP) and either 5 µg/mL, 10 µg/mL, or 25 µg/mL AOL. (B) Detection precision of DU145 MCTS treated with 50µM cyclo-RGDfK(TPP) and either 5 µg/mL, 10 µg/mL, or 25 µg/mL UEA-1.

Inline graphic — Comparison of the manual detection precisions of ≥40µm vs ≥60µm DU145 in the fucose lectin inhibition assay. Precision, p, is calculated using the formula, , expressed as a percentage. True positives are MCTS possess a spheroidal morphology²³^,²⁴ and diameter threshold (either ≥40 µm or ≥60 µm). False positives are MCTS that possess the spheroidal morphology but do not meet the diameter threshold. (A) Detection precision of DU145 MCTS treated with 50µM cyclo-RGDfK(TPP) and either 5 µg/mL, 10 µg/mL, or 25 µg/mL AOL. (B) Detection precision of DU145 MCTS treated with 50µM cyclo-RGDfK(TPP) and either 5 µg/mL, 10 µg/mL, or 25 µg/mL UEA-1.