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. 2019 Dec 19;11:10599–10608. doi: 10.2147/CMAR.S222681

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© 2019 Sun et al.

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The effects of miR-503 on expression levels of VEGFA in A172 and U251 cells, and cell proliferation, cell migration and angiogenesis of coculture HCMEC/D3 cells. (A) The miR-503 and VEGFA transcription levels were detected by qRT-PCR in A172 and U251 cells. (B) Western blot analysis of VEGFA expression in A172 and U251 cells. (C) ELISA assay was performed to detect VEGFA concentration using the cocultured system. (D) HCMEC/D3 proliferation induced by co-culturing with glioma cells was assessed by the MTT method using the cocultured system. (E) The migration ability of cocultured HCMEC/D3 cells was detected by transwell assay. (F) HCMEC/D3 angiogenesis ability in cells after indicated treatment. The experiment was repeated for three times. Data are the mean ± SD for three independent experiments. P value was determined by one-way ANOVA following Tukey's post hoc test. *P < 0.05; ** P < 0.01.