Figure 2.

An example of the physiological monitoring performed using the LabChart system from ADInstruments. For this experiment the chest motion respiratory monitor was used (pink channel 3) to provide a trigger signal (blue channel 4) that drives the shutter (green channel 8) and camera (orange channel 9) to acquire images at the same point in every breath. The aerosol detector (purple channel 11) confirms that treatment is delivered when the aerosol control signal (pink/red channel 10) is on. Note that (a) the ion chamber is placed before the fast shutter in this case, but can also be placed after the fast shutter to directly measure the dose to the animal; (b) it is not unusual for the animal to hold its breath briefly at the start of aerosol delivery.