KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat polyclonal anti-Robo3 (1:500 for IF) | R&D Systems | Cat#: AF3076; RRID: AB_2181865 |
| Mouse anti-Shh 5E1 (1:20 for IF) | Developmental Studies Hybridoma Bank | Cat#: 5E1; RRID:AB_528466 |
| Rabbit polyclonal anti-Arl13b (1:2000 for IF) | Caspary et al., 2007 | N/A |
| Mouse anti-Arl13b (1:2000 for IF) | NeuroMab | Cat# N295B/66; RRID:AB_2750771 |
| Rabbit polyclonal anti-IFT88 (1:1000) | kind gift from Brad Yoder | N/A |
| Mouse anti-Lhx2 (1:100 for IF) | Developmental Studies Hybridoma Bank | PCRP-LHX2-1C11; RRID:AB_2618817 |
| Donkey anti-goat IgG Cy3 (1:500) | Jackson ImmunoResearch Laboratory | Cat#: 705-165-147; RRID: AB_2307351 |
| Secondary antibodies were conjugated to Alexa Fluor 488, 568 or 594 (1:1000). | Thermo Fisher | Cat#: A21202; RRID:AB_141607, A10037; RRID:AB_2534013, A21206; RRID:AB_2535792, A21207; RRID:AB_141637 |
| Bacterial and Virus Strains | ||
| DH5a | Life Technologies | Cat#: 18265-017 |
| ccdB | Thermo Fisher | Cat#: A10460 |
| HB101 | Promega | Cat#: L2011 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Recombinant Human Sonic Hedgehog (C24II) N terminus | R&D Systems | Cat#: 1845-SH |
| Bovine serum albumin (BSA) | MultiCell | 500-0206 |
| Poly-L-lysine solution (molecular weight 70,000-150,000, concentration: 0.01%) | Sigma-Aldrich | P4707 |
| DAPI | Sigma-Aldrich | Cat# D9564 |
| Critical Commercial Assays | ||
| Primary Cell 96-well Nucleofector Kit | Lonza | Cat #: V4SP-3960 |
| Experimental Models: Organisms/Strains | ||
| Rat: ARS/Sprague Dawley | Charles River (St. Constant, Canada) | N/A |
| Arl13bhennin | Caspary et al., 2007 | MGI:3578151 |
| Tulp3tm1b(EUCOMM)hmgu | Legue and Liem, 2019 | MGI: 5548429 |
| Kif7 | Cheung et al., 2009 | MGI:4357956 |
| Arl13bV358A | Gigante et al., 2019 | MGI:6256969 |
| Recombinant DNA | ||
| pcDNA6.2-GW/EmGFP-miR | Invitrogen | K493600 |
| pCAGGs-mouse Arl13b-GFP | This article | N/A |
| pDONR221 | ThermoFisher | 12536-017 |
| pcDNA-DEST47 | ThermoFisher | 12281-010 |
| pCAGGS | Stühmer et al., 2002 | N/A |
| pCAGGS-Arl13bY86C-GFP | This article | N/A |
| pCAGGS-Arl13bR79Q-GFP | This article | N/A |
| pCAGGS-Arl13bR200C-GFP | This article | N/A |
| Software and Algorithms | ||
| Prism 7 for Mac OS X | Graphpad | https://download.cnet.com/GraphPad-Prism/3000-2053_4-8453.html |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |
| BLOCK-iT_ RNAi Designer | ThermoFisher Scientific | https://rnaidesigner.thermofisher.com/rnaiexpress/ |
| Illustrator CC | Adobe | http://www.adobe.com/jp/products/illustrator.html |
| Photoshop CC | Adobe | https://www.adobe.com/products/photoshop.html |
| Other | ||
| Dunn Chamber | Hawksley | DC-100 |