Table 1. Ingredients and proximate compositions of the experimental diets (as dry-matter basis %).
| Ingredients (g 100g−1) | Diets | |||||
|---|---|---|---|---|---|---|
| SPC0 | SPC15 | SPC 30 | SPC45 | SPC60 | SPC75 | |
| Fishmeal 60%(1) | 65.0 | 55.3 | 45.5 | 35.8 | 26.0 | 16.3 |
| Soy protein concentrate 65%(2) | 0.0 | 9.0 | 18.0 | 27.0 | 36.0 | 45.0 |
| Casein | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 | 5.0 |
| Shrimp meal | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 | 2.5 |
| Wheat flour | 18.0 | 17.8 | 17.5 | 17.3 | 17.0 | 16.8 |
| Binding agents | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 | 2.0 |
| Soybean oil | 1.0 | 1.5 | 2.0 | 2.5 | 3.0 | 3.5 |
| Fish oil | 1.0 | 1.5 | 2.0 | 2.5 | 3.0 | 3.5 |
| Squid visceral ointment | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| Vitamin premix(3) | 1.0 | 1.0 | 1.00 | 1.0 | 1.0 | 1.0 |
| Mineral premix(4) | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| Choline chloride | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| monocalcium phosphate | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 | 1.0 |
| Cr2O3 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 | 0.5 |
| Proximate composition | ||||||
| Crude protein | 46.56 | 46.49 | 47.11 | 46.12 | 46.85 | 46.3 |
| Crude lipid | 9.63 | 9.37 | 9.29 | 9.10 | 9.00 | 9.15 |
| Crude ash | 15.73 | 14.56 | 13.34 | 11.85 | 10.49 | 9.26 |
| ANFs (μg /100g) (5) | 0 | 0.68× 105 | 1.43 × 105 | 2.25× 105 | 2.96 × 105 | 3.50× 105 |
| Calculated gross energy (kJ g−1) | 0.079 | 0.078 | 0.079 | 0.079 | 0.081 | 0.081 |
(1) Fish meal: crude protein 60%, crude lipid 6%, ash 21% (dw), Lys 5.72%, Arg 4.31%, His 2.16%, Asp 6.28%, Glu 9.05%, Gly 4.18%, Ala 4.29%, val 2.41%, Leu 5.21%, Ile 2.40%, Phe 2.95%, Pro 2.37%, Trp 0.78%, Tyr 1.06%, Ser 2.66%, Met 2.23%, Thr 3.09%.
(2) Soy protein concentrate: crude protein 65.0%, crude lipid 1.0%, crude fiber 4.00%, ash 4.8%, soluble nitrogen-free extract 2.2% and insoluble nitrogen-free extract about 15% (dw), phytic acid 5.1 × 10 5 μg/100g, raffinos 1.1 × 105μg /100g, glycinin 9.04×104μg/100g, β-conglycinin 0 μg/100g, trypsin inhibitor 2.1×104μg/100g; Oligosaccharides, stachyose, lectins, sponins and urease activity cannot detected; Lys 4.06%, Arg 4.81%, His 1.54%, Asp 7.07%, Glu 13.55%, Gly 3.08%, Ala 3.11%, val 3.38%,Leu 5.24%, Ile 3.12%, Phe 3.23%, Pro 3.86%, Trp 0.97%, Tyr 2.35%, Ser 3.55%, Met 0.99%,Thr 2.62%.
(3) Vitamin mixture (mg kg−1 diet): retinol acetate, 38.0; cholecalciferol, 13.2; a-tocopherol, 210.0; thiamin, 115.0; riboflavin, 380.0 pyridoxine 88.0; pantothenic acid, 368.0; niacin acid, 1030.0 biotin, 10.0; folic acid, 20.0; vitamin B12, 1.3; inositol, 4000.0; ascorbic acid, 500.0 (Ding et al. 2010).
(4) Mineral mixture (mg kg−1 diet): MgSO4·7H2O, 3568.0; NaH2PO4·2H2O, 25568.0; KCl, 3020.5; KAl (SO4)2, 8.3; CoCl2, 28.0; ZnSO4·7H2O, 353.0; Ca-lactate, 15968.0; CuSO4·5H2O, 9.0; KI, 7.0; MnSO4·4H2O, 63.1; Na2SeO3, 1.5; C6H5O7Fe·5H2O, 1533.0; NaCl, 100.0; NaF, 4.0 (Ding et al. 2010).
(5) ANFs of diets: SPC0: 0; SPC 15: phytic acid 4.57 × 104 μg/100g, raffinos 0.99× 104 μg /100g, glycinin 1.1× 104 μg/100g, β-conglycinin 0 μg/100g, trypsin inhibitor 1100μg/100g; SPC30: phytic acid 0.90× 105 μg/100g, raffinose 0.20× 105 μg/100g, glycinin 3.02 × 104 μg/100g, β-conglycinin 0 μg/100g, trypsin inhibitor 3.1× 103 μg/100g; SPC45: phytic acid 1.36 × 105 μg/100g, raffinose 0.30 × 105μg/100g, glycinin 5.04 × 104 μg/100g, β-conglycinin 0 μg/100g, trypsin inhibitor 8.2 × 103 μg/100g; SPC60: phytic acid 1.82× 105μg/100g, raffinose 0.39× 105μg/100g, glycinin 6.03× 104 μg/100g, β-conglycinin 0 μg/100g, trypsin inhibitor 1.4 × 104μg/100g;SPC75: phytic acid 2.12 × 105μg/100g, raffinose 0.50 × 105μg/100g, glycinin 7.04 × 104 μg/100g, β-conglycinin 0 μg/100g, trypsin inhibitor 1.4× 104μg/100g. Oligosaccharides, stachyose, lectins, sponins and urease activity cannot be detect.