. 2019 Dec 23;14(12):e0222780. doi: 10.1371/journal.pone.0222780

Table 5. Amino acid composition of muscle (g kg⁻¹ dry weight for free amino acid and % for hydrolytic amino acid) of the juvenile pearl gentian grouper fed with diets containing various concentrations of FM replaced with SPC.

Items	Diets
	SPC0		SPC15		SPC 30		SPC45		SPC60		SPC75
	Hydrolytic	Free	Hydrolytic	Free	Hydrolytic	Free	Hydrolytic	Free	Hydrolytic	Free	Hydrolytic	Free
Polar basic amino acids
Arginine	3.81±0.04^d	0.77±0.01^a	4.11±0.02^a	0.31±0.01^c	3.95±0.03^bc	0.29±0.02^c	4.05±0.06^ab	0.77±0.01^a	3.90±0.05^cd	0.40±0.02^c	3.98±0.05^bc	0.65±0.02^b
Histidine	1.33±0.01^a	0.86±0.02^b	1.26±0.03^ab	0.99±0.03^a	1.25±0.03^ab	0.87±0.02^b	1.24±0.04^b	0.61±0.01^d	1.23±0.00^b	0.66±0.02^c	1.09±0.07^c	0.50±0.01^e
Lysine	6.30±0.15^b	4.93±0.10^a	6.57±0.08^a	4.40±0.02^b	6.26±0.05^b	3.68±0.01^c	6.25±0.04^b	3.32±0.02^d	6.29±0.12^b	3.10±0.01^e	5.92±0.14^c	1.22±0.02^f
Polar acidic amino acids
Aspartic acid	7.59±0.02^d	0.66±0.01^a	8.00±0.13^a	0.62±0.01^b	7.81±0.11^b	0.37±0.01^c	7.80±0.02^b	0.33±0.02^c	7.67±0.03^c	0.32±0.02^c	7.83±0.12^b	0.33±003^c
Glutamic acid	11.13±0.17^c	5.22±0.10^b	11.81±0.26^a	5.42±0.13^a	11.51±0.15^b	4.17±0.12^c	11.51±0.15^b	3.97±0.12^d	11.47±0.23^b	3.10±0.20^f	11.52±0.20^b	3.73±0.13^e
Non-polar amino acids
Isoleucine	1.93±0.03^a	1.83±0.01^a	2.05±0.04^b	1.67±0.04^b	1.97±0.04^ab	1.49±0.17^c	1.95±0.05^ab	1.17±0.21^d	2.00±0.03^ab	1.14±0.09^e	1.99±0.05^ab	1.15±0.15^de
Leucine	5.48±0.23^b	4.13±0.22^c	5.89±0.22^a	4.23±0.21^b	5.63±0.19^b	4.44±0.11^a	5.64±0.18^b	2.92±0.12^e	5.57±0.24^b	3.08±0.12^e	5.47±0.12^b	2.59±0.10^f
Phenylalanin	2.71±0.02^ab	2.16±0.17^a	2.83 ±0.02^a	2.11±0.12^a	2.70±0.05^ab	1.86±0.11^b	2.74±0.02^ab	1.42±0.10^d	2.72±0.03^ab	1.57±0.12^c	2.69±0.05^b	1.44±0.01^de
Valine	2.21±0.01^ab	2.86±0.01^a	2.28±0.14^a	2.63±0.02^b	2.21±0.02^ab	2.17±0.02^c	2.17±0.05^b	1.79±0.11^e	2.27±0.02^a	1.79±0.02^e	2.25±0.03^ab	2.03±0.10^d
Glycine	3.50±0.21^c	5.12±0.01^c	3.71±0.03^b	4.83±0.02^d	3.98±0.10^a	8.13±0.13^b	4.06±0.09^a	8.46±0.00^a	3.69±0.17^b	5.21±0.01^c	3.73±0.13^b	4.08±0.02^e
Alanine	4.87±0.30^b	4.88±0.01^a	4.94±0.05^ab	3.86±0.03^b	4.91±0.26^ab	3.73±0.13^b	4.99±0.26^a	3.78±0.13^b	4.89±0.15^ab	2.17±0.12^d	4.93±0.03^ab	3.18±0.12^c
Polar neutral amino acids
Methionine	1.95±0.03^b	0.92±0.01^b	1.94±0.03^b	0.84±0.02^c	2.06±0.04^a	1.52±0.01^a	1.94±0.02^b	0.87±0.02^c	1.98±0.02^b	0.51±0.02^d	1.75±0.03^c	0.28±0.01^e
Threonine	2.85±0.03^b	0.78±0.01^c	3.04±0.10^a	0.64±0.05^d	2.97±0.0^a	1.19±0.05^b	3.02±0.10^a	1.66±0.02^a	2.94±0.02^ab	0.71±0.01^c	2.94±0.05^ab	0.42±0.01^e
Serine	2.92±0.17^c	0.51±0.01^b	3.10±0.09^a	0.29±0.02^d	2.96±0.07^c	0.35±0.01^c	3.09±0.20^a	0.88±0.02^a	3.03±0.15^ab	0.38±0.01^c	2.99±0.06^ab	0.27±0.01^d
Tyrosine	2.13±0.12^b	1.41±0.01^a	2.23±0.25^a	0.33±0.01^d	2.11±0.19^b	0.50±0.02^c	2.10±0.21^b	0.83±0.01^b	2.10±0.17^b	0.91±0.04^b	2.16±0.32^b	1.08±0.02^b
Cysteine	0.51±0.03^b	0.14±0.01	0.53±0.02^ab	0.13±0.00	0.54±0.01^a	0.13±0.01	0.52±0.01^ab	0.13±0.01	0.52±0.05^ab	0.14±0.00	0.51±0.02^b	0.19±0.01
Taurine	0.58±0.02^a	4.85±0.11^a	0.39±0.01^c	4.65±0.20^b	0.42±0.01^c	4.09±0.10^b	0.40±0.06^c	3.96±0.14^d	0.51±0.02^b	4.08±0.12^b	0.49±0.02^b	4.62±0.02^b
ΣEAA	28.57±0.20^bc	19.25±0.07^a	29.97±0.16^a	17.82±0.04^b	28.99±0.17^b	17.50±0.10^b	29.00±0.21^b	14.53±0.04^c	28.57±0.09^bc	12.96±0.03^d	28.09±0.15^d	10.28±0.04^e
ΣNEAA	33.23±0.02^d	22.79±0.05^a	34.74±0.20^a	20.13±0.12^c	34.26±0.13^b	21.46±0.13^b	34.46±0.13^ab	22.33±0.15^a	33.89±0.05^c	16.84±0.15^d	34.16±0.14^bc	17.47±0.17^d
ΣAA	61.74±0.23^e	42.04±0.11^a	64.71±0.35^a	37.95±0.02^c	63.25±0.26^bc	38.97±0.12^b	63.46±0.33^a	36.87±0.04^d	62.76±0.11^cd	29.80±0.05^e	62.25±0.22^de	27.75±0.13^f
ΣDAA	27.09±0.03^e	15.88±0.01^c	28.50±0.13^a	14.73±0.04^d	28.22±0.14^bc	16.39±0.03^b	28.35±0.13^ab	16.54±0.02^a	27.72±0.02^d	11.34±0.02^e	28.02±0.10^c	11.31±0.04^e
ΣEAA/ΣAA	0.46±0.01	0.46±0.01^a	0.46±0.01	0.47±0.02^a	0.46±0.01	0.45±0.01^ab	0.46±0.01	0.39±0.01^c	0.46±0.01	0.43±0.01^ab	0.45±0.01	0.37±0.02^c
ΣEAA/ΣNEAA	0.86±0.02	0.84±0.01	0.86±0.01	0.88±0.02	0.85±0.01	0.82±0.01	0.84±0.02	0.65±0.02	0.85±0.02	0.77±0.02	0.82±0.01	0.59±0.02
ΣDAA/ΣAA	0.44±0.02	0.38±0.01	0.44±0.02	0.38±0.01	0.45±0.01	0.42±0.01	0.44±0.01	0.45±0.02	0.44±0.01	0.38±0.02	0.45±0.01	0.41±0.02

Values (mean ± SD, n = 3) within the same row with different letters are significantly different (P < 0.01). Absence of letters indicates no significant difference between treatments. ΣAA = total amino acid. ΣEAA = total essential amino acid. ΣNEAA = total nonessential amino acid. ΣDAA = delicious amino acid

Table 5. Amino acid composition of muscle (g kg−1 dry weight for free amino acid and % for hydrolytic amino acid) of the juvenile pearl gentian grouper fed with diets containing various concentrations of FM replaced with SPC.

Table 5. Amino acid composition of muscle (g kg⁻¹ dry weight for free amino acid and % for hydrolytic amino acid) of the juvenile pearl gentian grouper fed with diets containing various concentrations of FM replaced with SPC.