Table 7. Protein and individual essential amino acid (EAA) retention (%) in the muscle of the juvenile pearl gentian grouper fed with diets containing various concentrations of FM replaced with SPC.

Items	Diets
	SPC0	SPC15	SPC 30	SPC45	SPC60	SPC75
Protein	28.89 ± 0.17b	37.92 ± 1.69ab	39.28 ± 0.87a	24.77 ± 1.26c	26.02 ± 1.00c	18.07 ± 0.46d
Polar basic amino acid
Lysine	48.70 ± 0.58d	70.78 ± 2.61a	62.21 ± 2.31b	56.00 ± 0.61c	46.78 ± 0.32d	31.77 ± 0.76e
Arginine	30.85 ± 0.58c	41.15 ± 2.22a	36.72 ± 1.22b	32.05 ± 0.34c	24.54 ± 0.20d	17.27 ± 0.40e
Histidine	38.93 ± 0.58c	44.09 ± 2.22a	41.67 ± 1.12b	34.34 ± 1.10d	25.37 ± 0.14e	15.30 ± 0.68f
Non-polar amino acid
Isoleucine	32.55 ± 1.47cd	46.40 ± 3.95a	40.40 ± 2.50b	34.53 ± 1.11c	29.04 ± 1.31d	22.99 ± 2.36e
Leucine	32.02 ± 1.21d	47.52 ± 3.52a	41.75 ± 1.27b	35.94 ± 0.59c	28.40 ± 0.60e	20.48 ± 0.76f
Phenylalanine	32.06 ± 0.72c	43.19 ± 3.06a	36.93 ± 2.31b	31.18 ± 0.45c	23.70 ± 0.48d	16.69 ± 0.70e
Valine	31.53 ± 0.40c	44.58 ± 2.49a	39.86 ± 1.58b	33.63 ± 1.06c	30.03 ± 0.76c	21.20 ± 0.28d
Polar neutral amino acid
Methionine	43.45 ± 0.73d	75.09 ± 3.81a	64.41 ± 2.15b	51.76 ± 1.73c	50.08 ± 2.02c	37.30 ± 2.02e
Threonine	33.08 ± 0.28d	43.21 ± 1.75a	42.52 ± 0.92a	38.42 ± 0.81c	29.09 ± 0.80e	20.62 ± 1.51f

Values (mean ± SD, n = 3) within the same row with different letters are significantly different (P < 0.01). Absence of letters indicates no significant difference between treatment