Fig 2. Expression of specific markers of rhombic lip demonstrates that the most dorsal precursors were lost and that ventral markers were shifted dorsally in the caudal rhombic lip in Bmpr double knockout animals.

(A) Atoh1 expression in the caudal rhombic lip in normal animal at 10.5 dpc. Atoh1 expression was essentially lost in Bmpr double knockouts at the same stage (B). (C) Ngn2 expression in normal animals. (D) Ngn2 expression was shifted dorsally in the Bmpr double knockout animals (arrow marks the dorsal extent of expression in C, D). (E, F) Expression of Lhx1/5 and Pax2 demonstrate a vast reduction in the dorsal-most Lhx1/5-labled population (referred to as dA2 from reference 70; green arrow in F), and dorsal shift in class B neurons (orange arrow), which double-label for Lhx1/5 and Pax2, in the Bmpr mutant. Scale bar: A (for A-D), 50 μm; F (for E-F), 25 μm.