Figure 4.

5-Aza-2ʹ-deoxycytidine reversed cardiac hypertrophy and IRX1 downregulation in TAC-induced heart failure.

(a) qRT-PCR determined IRX1 mRNA expression in the left ventricles. (b-c) Western blot analysis determined IRX1 protein expression in the left ventricles. (d, f) Sirius red staining and morphometric analysis identified interstitial cardiac fibrosis. (e) TdT-mediated dUTP-biotin nick end labeling (TUNEL) staining to perceive detect apoptotic cells. (g) TUNEL positive cells ratio. Data are presented as the mean ± SD of five animals; **P < 0.01, ***P < 0.001, vs. Sham-Normal group, # P < 0.05, ## P < 0.01 vs. TAC-Normal group.