Table 1.
Primers used in this study.
| Gene |
|
Sequence (5’-3’) |
| IRX1 | Forward | GAAATCCTGGGCACAGTCCA |
| Reverse | CACAGTGGTTTTGCTCGGTG | |
| CXCL14 | Forward | GCAGGGCAATTGGGAACAAG |
| Reverse | GGGTGCAGGGTCATCCTAAC | |
| ANP | Forward | CCGGTACCGAAGATAACAGC |
| Reverse | CCGGTACCGAAGATAACAGC | |
| BNP | Forward | CTGGGAAGTCCTAGCCAGTCTCCA |
| Reverse | GCGACTGACTGCGCCGATCCGGTC | |
| α-MHC | Forward | ACAGAGTGCTTCGTGCCTGAT |
| Reverse | CGAATTTCGGAGGGTTCTGC | |
| β-MHC | Forward | GCTACCCAACCCTAAGGATGC |
| Reverse | TCTGCCTAAGGTGCTGTTTCA | |
| GAPDH | Forward | AACGACCCCTTCATTGACCT |
| Reverse | CCCCATTTGATGTTAGCGGG | |
| MSP(M) | Forward | TTTTTTTAAGAGTAAGGGGGTTAGC |
| Reverse | TAAAATATTCGAATAAATCAACGCT | |
| MSP(UM) | Forward | TTTTTAAGAGTAAGGGGGTTAGTGA |
| Reverse | TAAAATATTCAAATAAATCAACACT | |
| shIRX1-A | Forward | CACCGCCTGCTTATTATCCCTATGG CGAACCATAGGGATAATAAGCAGGC |
| Reverse | AAAAGCCTGCTTATTATCCCTATGG TTCGCCATAGGGATAATAAGCAGGC | |
| shIRX1-B | Forward | CACCGGAGAGCATTGACATCGATCA CGAATGATCGATGTCAATGCTCTCC |
| Reverse | AAAAGGAGAGCATTGACATCGATCA TTCGTGATCGATGTCAATGCTCTCC |
M: methylation primer; UM: unmethylation primer.