TABLE 6.
Results for the detection of Cry1Ac and PAT (bar) proteins in sugar samples from sugarcane stalks from the GM CTC91087-6 and non-GM CTC9001. Data for protein detection correspond to three technical ELISA assay replicates (n = 3). Samples harvest from Piracicaba (São Paulo State), 4 repetitions.
| Target | Assay | LOD | Lot Number** | Results |
|---|---|---|---|---|
| DNA | Probe Cry1Ac - Eurofins | 0.01% | 1 | NEGATIVE |
| DNA | Probe Cry1Ac - Eurofins | 0.01% | 2 | NEGATIVE |
| DNA | Probe Cry1Ac - Eurofins | 0.01% | 3 | NEGATIVE |
| DNA | Probe Cry1Ac - Eurofins | 0.01% | 4 | NEGATIVE |
| DNA | Probe PAT - Eurofins | 0.01% | 1 | NEGATIVE |
| DNA | Probe PAT - Eurofins | 0.01% | 2 | NEGATIVE |
| DNA | Probe PAT - Eurofins | 0.01% | 3 | NEGATIVE |
| DNA | Probe PAT - Eurofins | 0.01% | 4 | NEGATIVE |
| DNA | Event Specific Assay | 0.008 ng/mg sugar | 1 | < LOD |
| DNA | Event Specific Assay | 0.008 ng/mg sugar | 2 | < LOD |
| DNA | Event Specific Assay | 0.008 ng/mg sugar | 3 | < LOD |
| DNA | Event Specific Assay | 0.008 ng/mg sugar | 4 | < LOD |
| Protein | ELISA Cry1Ac | 0.0085µg/g sugar | 1 | < LOD |
| Protein | ELISA Cry1Ac | 0.0085µg/g sugar | 2 | < LOD |
| Protein | ELISA Cry1Ac | 0.0085µg/g sugar | 3 | < LOD |
| Protein | ELISA Cry1Ac | 0.0085µg/g sugar | 4 | < LOD |
| Protein | ELISA PAT | 0.006µg/g sugar | 1 | < LOD |
| Protein | ELISA PAT | 0.006µg/g sugar | 2 | < LOD |
| Protein | ELISA PAT | 0.006µg/g sugar | 3 | < LOD |
| Protein | ELISA PAT | 0.006µg/g sugar | 4 | < LOD |