Figure 4.

MiR-139-5p affected the biological functions of malignant melanoma cells by regulating IGF1R. (a), protein expression of IGF1R in cells of each group; (b-c), cell proliferation was evaluated by MTT assay and BrdU staining; (d), cell cycle distribution was detected with PI single staining; (e), cell apoptosis condition of groups was detected with Annexin V/PI double staining; (f-g), changes of cell migration and invasion capacity were, respectively, detected with Transwell assay; data were all measurement data and expressed as mean ± standard deviation; ANOVA was used for comparisons among multiple groups, followed by Tukey’s post hoc test;* P< 0.05vs the oe-NC group; # P< 0.05vs the oe-IGF1R group.