Figure 4.
HUCB-MSCs delivered exogenous miR-1246 via exosomes to alleviate hepatic IRI via GSK3β-mediated Wnt/β-catenin pathway. (a and b) The serum levels of ALT, AST at 6, 12 and 24 h post-surgery in C57BL/6 mice in the groups of Sham, IRI, PBS, Exo, NCI-Exo and miR-1246I-Exo. (c and d) hepatic histopathology and hepatocyte apoptosis of different groups using H&E staining and TUNEL assay (original magnifcation 200×); The protein levels of Wnt1, Wnt3a, GSK3β and β-catenin (e), the levels of TNF-α (f), IL-6 (g) and IL-1β (h) in livers obtained from different groups. *P < 0.05 vs. NCI-Exo; #P < 0.05 vs. Sham; &P < 0.05 vs. PBS. n = 6.