Figure 1.

miR-219 is upregulated in liver CSCs.

a. C133 positive primary HCC cells were sorted and subjected to real-time PCR assay (n = 3). b. OV6 positive primary HCC cells were sorted and subjected to real-time PCR assay (n = 3). c. Real-time PCR analysis of miR-219 in CD133 positive HCC cells and control negative HCC cells (n = 3). d. Real-time PCR analysis of miR-219 in OV6 positive HCC cells and control negative HCC cells (n = 3). e. Real-time PCR analysis of miR-219 in primary HCC adherent and spheroids cells (n = 3). f. Real-time PCR was performed to examine the expression of miR-219 in hepatoma spheroids (n = 3). g. HCCLM3 and hepG2 cell–derived spheroids were trypsinized and cultured in attachment conditions. miR-219 expression in spheroids versus reattached cells was compared by real-time PCR (n = 3).(Data are represented as mean±s.d.; *P < 0.05; two-tailed Student’s t-test.)