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. 2019 Oct 15;18(23):3288–3299. doi: 10.1080/15384101.2019.1673633

Figure 4.

Figure 4.

MiR-223 directly targets HSP90B1 in BC cells. (a) Bioinformatics analysis showed that miR-223 could directly target the HSP90B1 3′-UTR. (b) Exploring miR-223 expression in several human cancers using the BioXpress database. Bladder cancer: BLCA. (c) Prognostic value of HSP90B1 mRNA expression in BC patients was analyzed using the KM lotter database. (d) Expression of HSP90B1 mRNA in human BC cell lines (T24 and SW780) and human normal human urothelial cell line SV-HUC-1. (e) The luciferase-reporter plasmid containing WT or mutant HSP90B1 3′-UTR was co-transfected into BC cells, along with miR-223 mimic or miR-223 inhibitor as indicated. Then, the relative luciferase activity was performed by dual-luciferase reporter assays. (f) The protein expression of HSP90B1 was examined in BC cells transfected with miR-223 mimic or miR-223 inhibitor as indicated. *P < 0.05.