Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Antibody | mouse monoclonal anti-PanNav | Sigma | clone K58/35 CAT#S8809 RRID:AB_477552 |
(1:500) |
| Antibody | rabbit polyclonal anti-Nav1.6 | Alomone | Cat # ASC-009 RRID:AB_2040202 |
(1:200) |
| Antibody | rabbit polyclonal anti-KCC2 | Millipore | CAT# 07–432 RRID:AB_310611 |
(1:400, 1:500) |
| Antibody | rabbit polyclonal anti-calpain-I | Ozyme | CAT#2556S | (1:500) |
| Antibody | rabbit polyclonal anti-calpain-II | Millipore | CAT#AB81023 RRID:AB_1586917 |
(1:500) |
| Antibody | ImmunoPure goat HRP-conjugated mouse-specific antibody | Thermo Fisher Scientific | CAT#0031430 RRID:AB_228307 |
(1:40000) |
| Antibody | AlexaFluor-488 goat anti-mouse IgG2b | Thermo Fisher Scientific | CAT# A-21141 RRID:AB_141626 |
(1:800) |
| Antibody | AlexaFluor-546 F(ab’)two goat anti-rabbit IgG | Thermo Fisher Scientific | CAT# A-11071 RRID:AB_2534115 |
(1:400) |
| Chemical compound, drug | NaCl | Sigma-Aldrich | CAT# 71376 | |
| Chemical compound, drug | KCl | Sigma-Aldrich | CAT# P3911 | |
| Chemical compound, drug | NaH2PO4 | Sigma-Aldrich | CAT# S0751 | |
| Chemical compound, drug | MgSO4 | Sigma-Aldrich | CAT# 1880 | |
| Chemical compound, drug | CaCl2 | Sigma-Aldrich | CAT# 21115 | |
| Chemical compound, drug | NaHCO3 | Sigma-Aldrich | CAT# S6014 | |
| Chemical compound, drug | D-glucose | Sigma-Aldrich | CAT# G8270 | |
| Chemical compound, drug | K+-gluconate | Sigma-Aldrich | CAT# P1847 | |
| Chemical compound, drug | MgCl2 | Sigma-Aldrich | CAT# M8266 | |
| Chemical compound, drug | HEPES | Sigma-Aldrich | CAT# H3375 | |
| Chemical compound, drug | EGTA | Sigma-Aldrich | CAT# E3889 | |
| Chemical compound, drug | ATP | Sigma-Aldrich | CAT# A9062 | |
| Chemical compound, drug | GTP | Sigma-Aldrich | CAT# G9002 | |
| Chemical compound, drug | Sucrose | Sigma-Aldrich | CAT# S9378 | |
| Chemical compound, drug | Cadmium Chloride | Sigma-Aldrich | CAT# 202908 | |
| Chemical compound, drug | Tetraethylammonium chloride | Sigma-Aldrich | CAT# 86616 | |
| Chemical compound, drug | Nifedipine | Sigma-Aldrich | CAT# N7634 | |
| Chemical compound, drug | Dimethylsulphoxide | Sigma-Aldrich | CAT# D8418 | |
| Chemical compound, drug | Paraformaldehyde | EMS | CAT# 15714 s | |
| Chemical compound, drug | Phosphate Buffered Saline | Argene Biomérieux | CAT# 33–011 | |
| Chemical compound, drug | Tissue-Tek OCT compound | VWR | CAT# 25608–930 | |
| Chemical compound, drug | Tris-buffered saline | Bio-world | CAT# 105300272 | |
| Chemical compound, drug | Triton X-100 | Sigma-Aldrich | CAT# T9284 | |
| Chemical compound, drug | CompleteMini | Roche diagnostic Basel | CAT#11836170001 | |
| Chemical compound, drug | Iodoacetamide | Sigma-Aldrich | CAT#I1149 | |
| Chemical compound, drug | Igepal CA-630 | Sigma-Aldrich | CAT#I8896 | |
| Chemical compound, drug | SDS | Sigma-Aldrich | CAT#05030–1 L-F | |
| chemical compound, drug | Acryl/Bisacrylamide solution | Biorad | CAT#161–0146 | |
| Chemical compound, drug | PMSF | Sigma-Aldrich | CAT#93482 | |
| Chemical compound, drug | Pepstatin A | Sigma-Aldrich | CAT#77170 | |
| Chemical compound, drug |
MDL28170 | Calbiochem | CAT#208722 | |
| Chemical compound, drug | Calpain 1 | Calbiochem | CAT#208712 | |
| Chemical compound, drug |
Bay K8644 | Sigma-Aldrich | CAT# B112 | |
| chemical compound, drug |
CNQX | Sigma-Aldrich | CAT# C127 | |
| Chemical compound, drug |
DIOA | Sigma-Aldrich | CAT# D129 | |
| Chemical compound, drug | Veratridine | Sigma-Aldrich | CAT# V5754 | |
| Chemical compound, drug |
AP5 | Tocris | CAT# 3693/10 | |
| Chemical compound, drug |
PCPz | Tocris | CAT# 3287/100 | |
| Chemical compound, drug |
Riluzole | Tocris | CAT# 0768/25 | |
| Chemical compound, drug | 4,9-ah-TTX | Focus Biomolecules | CAT# 10–3700 | |
| Strain, strain background (Rattus norvegicus) | Wistar rats | Charles River Laboratories | RRID:RGD_2308816 | |
| Software, algorithm | pClamp v10.3 | Molecular Devices | RRID:SCR_011323 | |
| Software, algorithm | ImageJ v1.50i | https://imagej.nih.gov/ij/ | RRID:SCR_003070 | |
| Software, algorithm | FluoView v5.0 | Olympus | RRID:SCR_014215 | |
| Software, algorithm | Image Lab v5.1 | Bio-Rad | RRID:SCR_014210 | |
| Software, algorithm | Graphpad Prism | Prism | RRID:SCR_002798 | |
| Other | Vibrating microtome | Leica | VT1000S RRID:SCR_016495 |
|
| Other | Temperature controller | Warner Instruments | CL-100 | |
| Other | Nikon Eclipse microscope | Nikon | E600FN | |
| Other | Confocal microscope | Zeiss | LSM510 | |
| Other | Infrared-sensitive CCD camera | Hitachi | KP-200/201 | |
| Other | Digidata 1440a interface | Molecular Devices | N/A | |
| Other | Multiclamp 700B amplifier | Molecular Devices | N/A | |
| Other | Borosilicate glass capillaries | World Precision Instruments | CAT# TW150-4 | |
| Other | Sutter P-97 puller | Sutter Instruments | RRID:SCR_016842 | |
| Other | Knittel Glass coverslips | Dutscher | CAT# 900529 | |
| Other | Polysine slides | Thermoscientific | CAT# P4981 | |
| Other | Small Animal Ventilator | CWE | SAR-830/AP | |
| Other | Cardiotachometer | CWE | CT-1000 | |
| Other | Chemidoc imaging system XRS+ | Bio-rad | RRID:SCR_014210 |