Table 2. Summary of Ig sequences, clonal families and their corresponding mean somatic hypermutation (SHM) in unstimulated PBMCs vs. stimulated PBMCs from patient 013.
| Unstimulated PBMCs | Stimulated PBMCs | |
|---|---|---|
| VH all unique reads | 162928 | 1245789 |
| VH sequences with UMI ≥ 2 | 18588 | 146287 |
| VH clonal families | 11596 | 11407 |
| IgM sequences | 15318 | 4684 |
| SHM, IgM sequences | 0.6% | 2.7% |
| IgG sequences | 2156 | 129636 |
| SHM, IgG sequences | 6.7% | 7.0% |
| IgA sequences | 1114 | 11967 |
| SHM, IgA sequences | 7.0% | 6.2% |
| Clonal family threshold* | 17.8% | 21.4% |
| IgM clonal families | 9794 | 2260 |
| SHM, IgM clonal families | 0.6% | 1.9% |
| IgG clonal families | 1243 | 5657 |
| SHM, IgG clonal families | 6.0% | 5.6% |
| IgA clonal families | 559 | 3490 |
| SHM, IgA clonal families | 6.9% | 6.3% |
VH = variable heavy chain, VL = variable light chain, UMI = unique molecular identifier. * Nucleotide distance used to group related sequences into clonal families.