Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HeLa | Institute Curie, Paris, France | ||
| Cell line (Homo sapiens) | HEK293T | ATCC | CRL-1168 | |
| Strain, strain background (Mus musculus) | WT | Jackson Laboratory | C57BL/6J | Provided by UAB animal facility |
| Strain, strain background (Mus musculus) | Cpt1c KO | DOI:10.1074/jbc.M111.337493 | B6129SvEv/C57BL/6J Cpt1c-/- | Exons deleted: 12–15. Generated at UAB-CBATEG. 8 times backcrossed to C57BL/6J |
| Transfected construct (M. musculus) | EV | Addgene | #12254; pWPI-IRES-GFP | Empty vector. Lentiviral construct |
| Transfected construct (M. musculus) | CPT1C | DOI:10.1016/j.molmet.2018.10.010 | pWPI-CPT1C-IRES-GFP | |
| Transfected construct (M. musculus) | CPT1CM589S | DOI: 10.1016/j.molmet.2018.10.010 | pWPI-CPT1CM589S-IRES-GFP | |
| Transfected construct (M. musculus) | CPT1CR37C | This paper | pWPI-CPT1CR37C-IRES-GFP; | Lentiviral construct to express mutated CPT1CR37C (c.109C > T). |
| Transfected construct (M. musculus) | shProtrudin | Tebu-Bio | Psi-LVRU6MP-shProtrudin | Cloned sequence: 5’-agaatgaggtgctgcgcag-3’ |
| Transfected construct (H. sapiens) | EGFP-FYCO1 | DOI: 10.1038/nature14359 | pDEST-EGFP-FYCO1 (AmpR) | |
| Transfected construct (H. sapiens) | CPT1C-mTurquoise2 | other | Empty vector: #54843 | Vector from Addgene. Clonning at D Serra’s lab |
| Transfected construct (H. sapiens) | mTurquoise-calnexin | Addgene | #55539 | |
| Transfected construct | mTurquoise-ER | Addgene | #55550 | |
| Transfected construct | mCitrine-ER-5 | Addgene | #56557 | |
| Transfected construct (H. sapiens) | mCherry-FYCO1 | other | pDestmCherry-C1-FYCO1 | From T Johansen’s lab |
| Transfected plasmid (H. sapiens) | mCitrine-Protrudin | DOI: 10.1074/jbc.M112.419127 | From WD Heo’s lab | |
| Transfected plasmid (R. norvegicus) | Myc-CPT1C | This paper | pCDNA3.1-Myc-His-CPT1C | Empty vector from ThermoFisher:V80020 |
| Antibody | Anti LAMP1 (rabbit polyclonal) | Sigma | L1418 | IF: (1:250) |
| Antibody | Anti Myc (mouse monoclonal) | ATCC | 9E10 | IF: (1:50) West: (1:80) |
| Antibody | Anti KIF5B (rabbit monoclonal) |
Abcam | ERP10277B; ab167429 | IF: (1:100) West (1:500) |
| Antibody | Anti GFP (rabbit monoclonal) | Cell Signaling | #2956 | West: (1:500) |
| Antibody | Anti protrudin (rabbit polyclonal) | PTG | 12680–1-AP | IF: (1:300) |
| Antibody | Anti Rab7 (rabbit monoclonal) | Cell Signaling | D95F2;9367 | IF: (1:60) |
| Antibody | Anti CPT1C (rabbit polyclonal) |
DOI: 10.1074/jbc.M707965200 | West: (1:2000) | |
| Antibody | Anti pACC (rabbit polyclonal) | Cell Signaling | #3661 | West: (1:1000) Against Ser79 |
| Antibody | Anti ACC (rabbit monoclonal) | Cell Signaling | #3676 | West: (1:1000) |
| Antibody | Anti SMI312 (mouse monoclonal) |
Convance | # SMI312R | IF: (1:1000) |
| Software | Imaris | Bitplane | BPA-IM91-CL | Imaris 9.1 for Cell Biologists |