Table 2.

Characterization of Fc gamma receptor binding by SPR at 25°C.

	FCGR1		FCGR2		FCGR3		FCGR4
	KD (nM)	N	KD (nM)	N	KD (nM)	N	KD (nM)	N
CD3 mG1 D265A	nb	8	lb	4	lb	4	lb/nb	4
CD3/CD20 mG1 D265A bsp	nb	12	lb	6	lb	6	lb/nb	6
CD3 mG2a	21 ± 3	12	1570 ± 438	6	625 ± 87	6	18 ± 4	5
CD3/CD20 mG2a bsp	33 ± 8	12	1993 ± 538	6	1030 ± 157	6	39 ± 22	6
CD3 mG2a D265A	nb	8	lb	4	lb	4	lb/nb	4
CD3/CD20 mG2a D265A bsp	nb	8	lb	4	lb	4	lb/nb	4

KD values ± standard deviation shown. N: number of measurement spots (ROIs) used; “lb”: low binding; “nb”: no binding.