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Characterization of CD3/CD20 bispecific antibodies by differential scanning calorimetry.
| Molecule | T Onset (°C) | Tm1 (°C) | Tm2 (°C) | Tm3 (°C) |
|---|---|---|---|---|
| CD3/CD20 mG1 D265A bsp | 55.3 | 63.6 | 67.6 | 79.6 |
| CD3 mG1 | 59.7 | 66.5 | 68.6 | 78.9 |
| CD20 mG1 | 63.8 | 72.9 | 76.1 | - |
| CD3/CD20 mG2a bsp | 54.8 | 67.7 | 71.6 | 75.6 |
| CD3 mG2a | 58.1 | 65.7 | 67.7 | 76.0 |
| CD20 mG2a | 60.4 | 71.5 | 75.2 | - |
