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. Author manuscript; available in PMC: 2020 May 29.
Published in final edited form as: Science. 2019 Oct 24;366(6469):1129–1133. doi: 10.1126/science.aay3965

Fig. 2. ParB CTP hydrolysis.

Fig. 2

(A) CTP hydrolysis measured by colorimetric detection of inorganic phosphate using malachite green. Mean values from three repeat measurements. Data points are shown as dots. (B) CTP hydrolysis by ParBBsu dimers with increasing DNA concentrations. Data points and fits of three repeat measurements. (C) Membrane-spotting showing parS-stimulated cooperative CTP binding. (D, E) Crystal structure of a ParBBsu(21-218)/CDP/Ca2+ dimer. (F) Catalytic center in ParBBsu(21-218)/CDP/Ca2+.