Extended Data Fig. 9. Depletion of PKR and GCN2 kinase is compensated over time.

(a) Immunoblots of APC^def cells upon knockdown of MYC, representative of two independent experiments with similar results. siRNA transfections were carried out using siCTR as non-targeting control or siMYC for 72 h. As positive control for PKR activation, cells were treated with poly(I:C) (2 μg/ml, 4 h).

(b) Crystal violet staining of shCTR-transduced, PKR- or GCN2-depleted APC^def and APC^res (six days ethanol or doxycycline, respectively), representative of three independent experiments with similar results. Two independent shRNAs for both PKR and GCN2 were used.

(c) Immunoblots of shCTR-transduced, PKR- or GCN2-depleted APC^def and APC^res cells (96 h ethanol or doxycycline, respectively), representative of three independent experiments with similar results.

Unprocessed immunoblots are shown in Source Data Extended Data 9.