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. 2019 Dec 17;7:430. doi: 10.3389/fbioe.2019.00430

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Copyright © 2019 Janjić, Samiei, Moritz and Agis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Characterization of human dental pulp cells (DPC). DPC under normoxia (A) and hypoxia (B) were stained for alkaline phosphatase. Staining was quantified and is displayed normalized to the normoxic control (E). Metabolic activity under normoxia and hypoxia was measured in parallel after 7 days (F). After 14 days, DPC were stained with alizarin red for calcium deposition under normoxia (C) and hypoxia (D) and quantified normalized to the normoxic control (G). Metabolic activity was measured at the same time under normoxia and hypoxia (H). Bars represent mean + standard deviation. N = 6. *p < 0.05 relative to normoxic control.