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. 2019 Dec 17;10:2890. doi: 10.3389/fmicb.2019.02890

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Copyright © 2019 Palusinska-Szysz, Luchowski, Gruszecki, Choma, Szuster-Ciesielska, Lück, Petzold, Sroka-Bartnicka and Kowalczyk.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Images illustrating Förster type excitation energy transfer (FRET) between SYTO9 immobilized on the surface of L. pneumophila cells (TF3/1 strain) and Nile Blue situated within A. castellanii cells. The left panel shows a fluorescence image of A. castellanii based on selective emission of Nile blue. The middle panel shows a map of FRET efficiency (L. pneumophila cells appear as bright spots). The right panel shows the FRET efficiency histogram representing an analysis of the map shown in the middle panel. The average FRET efficiency calculated over histograms in 6 experiments is given ± SD.