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. 2019 Dec 17;7:338. doi: 10.3389/fcell.2019.00338

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Copyright © 2019 Zhang, Winkler, Schlottmann, Kohlbacher, Elias, Skotheim and Ewald.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phospho-proteomics time course of yeast cells released synchronously from G1 on ethanol minimal medium. (A) Experimental workflow for sampling, phospho-enrichment, TMT labeling and mass spectrometry analysis. (B) Budding index of two replicate cultures released from a G1 arrest. (C) Total protein, and (D) Phosphorylated sites quantified in the two replicate experiments. (E) Heatmap of the averaged replicates (log₂ fold changes relative to t = 0 min) for phosphorylated sites and quantified proteins.