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. 2019 Dec 17;10:2891. doi: 10.3389/fmicb.2019.02891

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Copyright © 2019 Chang, Hou, Pan, Huang, Han, Bin, Deng, Li, Ding, Gao, Zhi, Yang and Bi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) The proportion of bacteria missed in different groups at a single time point and over the entire time period. Results are shown as means ± SEM. (B) The number of bacteria not isolated from the sample taken at the previous time point compared with the number of bacteria isolated from the current sample. (C) The change in bacterial identification rate over time. (D) Equation fitted to the number of bacterial species depending on the workload before and after optimization of the method.