FIGURE 2.

Phage HEf13 belongs to the Siphoviridae family. (A–C) Phage HEf13 (1 × 10⁸ PFU) was dropped onto a Formvar/carbon-coated copper grid and then negatively stained with 2% uranyl acetate. (D) E. faecalis KCOM 1162 (1 × 10⁸ CFU/mL) incubated with phage HEf13 (1 × 10¹⁰ PFU/mL) was dropped onto copper grids and stained with 2% uranyl acetate. Electron micrographs of phage HEf13 were obtained by TEM at (A) 35,000×, (B,C) 100,000×, and (D) 35,000× magnification. Identification and classification of phages was conducted according to ICTV guidelines. Scale bar indicates 100 nm (B,C) or 0.5 μm (A,D). Arrows in panel (D) show phage HEf13 binding to the cell wall membrane of E. faecalis KCOM 1162.