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Phage HEf13 was classified as the Sap6virus lineage. The amino acid sequences of the phage portal protein, tail fiber protein, and DNA methyltransferase of phage HEf13 were compared with those of other phages using the BioEdit Sequence Alignment Editor program. A phylogenetic tree for each phage protein was generated using MEGA7 software. Scale bars represent a 10 (A,B) or 5% (C) difference in sequence similarity between phages.
