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. 2019 Dec 17;10:1445. doi: 10.3389/fphys.2019.01445

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Copyright © 2019 Weng, Wu, Kao, Chen and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibition of miR-34a-5p promotes ADSC migration in vitro by up-regulating CTRP9. (A,B) Cell migration after transfection detected by the scratch test. (C,D) Cell migration after transfection detected by Transwell assay (200 ×). ^*p < 0.05 vs. the vector group; ^#p < 0.05 vs. the sh-NC group; ^&p < 0.05 vs. the miR-34a-5p inhibitor NC group; ^@p < 0.05 vs. the miR-34a-5p inhibitor group; ^$p < 0.05 vs. the DMSO group; ^%p < 0.05 vs. the miR-34a-5p inhibitor + DMSO group. Data (mean ± s.d.) among multiple groups were analyzed by one-way ANOVA, followed by Tukey’s post hoc test. Log-rank was used for univariate analysis. The experiment was repeated three times. CTRP9, C1q/tumor necrosis factor-related protein-9; miR-34a-5p, microRNA-34a-5p; ADSC, adipose-derived stem cell; sh, short hairpin; NC, negative control; DMSO, dimethyl sulfoxide; s.d., standard deviation; ANOVA, analysis of variance.