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. 2019 Dec 23;9:19645. doi: 10.1038/s41598-019-56036-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Relative RAC1 3′UTR luciferase activity in HepG2 cells treated with miRNAs. (A–H) Relative luciferase activity of the RAC1 3′UTR in HepG2 cells lines transfected with miR-134/miR-142/miR-155/miR-182/miR-192/miR-194/miR-218/miR-636. (I) Reporter gene vector map and construction. (J) Identification of AURKAPS1 and RAC1 3′utr reporter recombinant plasmids. Luciferase activity of the RAC13′UTR was examined by luciferase reporter gene assay. *P < 0.05, **P < 0.01. The means ± SD are shown. Statistical analysis was conducted using Student’s t-test.