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. 2019 Dec 17;13:539. doi: 10.3389/fncel.2019.00539

FIGURE 1.

FIGURE 1

Ablation of Rack1 in Purkinje cells (PCs) delays cerebellar lobule VII formation. (A) Ai9 reporter mice showed that Pcp2-Cre is specifically expressed in the PC layer of cerebellar cortex. Scale bar = 500 μm. (B) The expression of Pcp2-Cre was determined in the offspring of Pcp2-Cre;Ai9 mice. Cerebellar sections were counterstained with Calbindin antibody. Scale bar = 100 μm. (C) Representative Western blot shows the expression of Rack1 in the postsynaptic density (PSD) fraction of cerebellar lysates from P7 wild-type and Pcp2-Cre;Rack1F/F mutant mice. (D) Quantitative analysis of Western blot displays significant decreased expression of Rack1 in Pcp2-Cre;Rack1F/F mutants compared to control littermates. Mean ± SEM, ∗∗p = 0.0009, n = 5. (E) The body size of Pcp2-Cre;Rack1F/F mutant mice was indistinguishable compared to control littermates at P30. (F) Coimmunofluorescent staining with Rack1 and Calbindin antibodies show the significantly decreased expression of Rack1 in Purkinje cells in Pcp2-Cre;Rack1F/F mutants compared to control littermates. Scale bar = 100 μm. (G) Nissl staining of sagittal sections of the cerebellar vermis shows specific deficiency in cerebellar lobule VII foliation in Pcp2-Cre;Rack1F/F mutants compared to control littermates at P14, P21 but not at P60. Scale bar = 1 mm. (H) Quantitative analysis of the area of each individual lobule of the vermis in Pcp2-Cre;Rack1F/F mutant mice and control littermates at indicated developmental stages. ∗∗p = 0.007 and 0.008, at P14 and P21, respectively; p = 0.55 at P60, n = 5, n.s. = not significant.