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. 2019 Dec 17;12:306. doi: 10.3389/fnmol.2019.00306

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Copyright © 2019 Peña-Díaz, Pujols, Conde-Giménez, Čarija, Dalfo, García, Navarro, Pinheiro, Santos, Salvatella, Sancho and Ventura.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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PMCA of α-synuclein in presence of ZPD-2. (A) Tricine–SDS-PAGE gels of untreated (middle) and ZPD-2-treated (right) PMCA samples before (–) and after (+) being digested with proteinase K. (B) Th-T fluorescence of different PMCA cycles of treated (green) and untreated (black) samples. Soluble α-Syn and PMCA steps 4 and 5 are shown. Th-T fluorescence is plotted as normalized means. Error bars are shown as standard errors of mean values.