TABLE 2.
qPCR reaction cycle settings.
| Enzyme activation | 95°C | 10 min | |
| Cycle parameters | 95°C | 15 s | Denature |
| Repeat 40x | 48°C | 30 s | Anneal |
| 70°C | 30 s | Extend | |
| Melt curve | 70°C–95°C | Read every 0.5°C |
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