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. 2019 Dec 23;9:19703. doi: 10.1038/s41598-019-56145-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Pao extracts downregulated the expression of AR, SRD5A1, and PSA. (a) IHC staining of AR in the sham control, BPH/Veh, BPH/FN and BPH/Pao groups. Scale bar, 50 μm. (b) Quantification of AR positive area in four groups. (c) Western blot analysis of AR, SRD5A1, and PSA protein expression in rat prostate tissues. (d) Quantification of AR, SRD5A1, and PSA protein expression levels in rat prostate tissues from four groups by Image J software. The values were presented as the mean ± SD of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.