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. 2019 Dec 17;10:2913. doi: 10.3389/fimmu.2019.02913

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Copyright © 2019 Doz-Deblauwe, Carreras, Arbues, Remot, Epardaud, Malaga, Mayau, Prandi, Astarie-Dequeker, Guilhot, Demangel and Winter.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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rBCG::PGL-I preferentially triggers Syk/calcineurin/NFATc through CR3 to rewire innate cells (A) DCs or PMNs from WT or myd88^−/− mice were infected with the three rBCG strains at MOI of 5. WT cells were treated with GS-9973 to inhibit the Syk pathway, or CsA to block NFATc translocation 1 h before infection. After overnight incubation supernatants were harvested to measure by ELISA IL-2 produced by DCs and IL-10 produced by PMNs. (B) Before infection of DCs or PMNs with rBCG::noPGL or rBCG::PGL-I as in (A), cells were either incubated for 1 h with anti-CD11b antibody M1/70 to block CR3-mediated entry, or exposed to CsA to block NFATc translocation as indicated. IL-2 produced by DCs and IL-10 by PMNs after overnight incubation were measured by ELISA. Data are presented as mean ± SEM (n = 4). ^*P < 0.05; ^**P < 0.01; ^***P < 0.001.