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. 2019 Dec 17;10:2936. doi: 10.3389/fimmu.2019.02936

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Copyright © 2019 Toor, Murshed, Al-Dhaheri, Khawar, Abu Nada and Elkord.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immune checkpoints co-expression on CD4⁺ T cells. PBMC, NILs, and TILs isolated from CRC patients were stained for CD3, CD4, and immune checkpoints. Representative flow cytometric and scatter plots show differences in co-expression levels of PD-1^−/+CTLA-4^−/+ (A), PD-1^−/+TIM-3^−/+ (B), and PD-1^−/+LAG-3^−/+ (C) on CD4⁺ T cells in PBMC, NILs, and TILs. ^***P < 0.001.