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. 2019 Dec 17;10:1255. doi: 10.3389/fgene.2019.01255

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Copyright © 2019 Lin, Lin, Yin, Zhu, Yang, Shen, Yang, Chen, Hu, Ma, Shi, Shen, Hu, Huang and Huang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Design of the Agena iPLEX spinal muscular atrophy (SMA) assay. The iPLEX assay consists of a target-specific PCR reaction, followed by single-base extension using molecular weight-modified dideoxynucleotide terminators of an extension primer which anneals immediately upstream of the polymorphic site of interest two well-known positions of SMN1 and SMN2 genes (c.840 and c.1155) were used as targets for designing PCR and single-base extension primers. Black and blue colors indicate multiplex PCR and extension primers, respectively.