Fig. 1. LINC00703 interacts with miR-181a. (A) A schematic representation of LINC00703 location at chromosome 10 and 2 putative binding sites for miR-181a in LINC00703. (B) The luciferase reporter plasmid (KLF6 3′-UTR) was co-transfected with miR-181a and LINC00703-overexpressing plasmids into HEK-293T cells. The relative luciferase activity was measured 48h after transfection. (C) RIP with human monoclonal anti-Ago2, mouse preimmune IgG or 10% input from BGC-823 cell extracts. The qRT-PCR was performed to determine the RNA levels of miR-181a and LINC00703 in immunoprecipitates as compared to the input. (D) The expression of LINC00703 enriched by RNA pull-down assay. All data are presented as means±standard deviation (n=3, biological replicates).

LINC00703 = long noncoding RNA 00703; KLF = Kruppel-like factor; UTR = untranslated region; RIP = RNA binding protein immunoprecipitation; IgG = immunoglobulin G; qRT-PCR = quantitative real-time polymerase chain reaction.

^*P<0.05, ^†P<0.01, and ^‡P<0.001.