Fig. 2. LINC00703 regulates KLF6 expression. (A) The qRT-PCR analysis, demonstrating LINC00703 expression in several gastric cancer cell lines (MGC-803, SGC-7901, and BGC-823), compared with normal gastric epithelium cell line (GES-1). (B) LINC00703 expression analysis in BGC-823 cells, transfected with pcDNA3.1 or LINC00703-overexpressing vector (LINC00703). (C and D) Expression levels of miR-181a and KLF6 after 48 hours, following the transfection of pcDNA3.1 or LINC00703 in BGC-823 cells. (E) The KLF6 protein level in BGC-823 cells, transfected with miR-181a mimic, LINC00703 or both miR-181a mimic and LINC00703. All data are presented as means±standard deviation (n=5, biological replicates).

LINC00703 = long noncoding RNA 00703; KLF = Kruppel-like factor; qRT-PCR = quantitative real-time polymerase chain reaction.

^*P<0.05 and ^†P<0.01.